Akemi Negishi (根岸明美 Negishi Akemi, b. 26 March 1934, Tokyo, Japan - died 11 March 2008 (73), Kawasaki, Kanagawa, Japan) was a Japanese actress.

In 1953, when Josef von Sternberg made a film adaptation of the Queen of Anatahan incident, she was discovered at an audition and made her debut in the lead role in Anatahan. Since then, she has been active mainly in film and television. She gained popularity as an actress with a bewitching charm who "could act as well." Ishirō Honda said that Negishi's unique aura suited his films. She died of ovarian cancer at a hospital in Kawasaki City on March 11, 2008. She was 73 years old.